The　development　of　inexpensive　and　highly　efficient　enzyme-responsive　polymers　has　significantly　contributed　to　targeted　drug　delivery　systems.　Here,　a　superamphiphile　with　a　capability　of　fluorescent　dissociation　sensing　is　designed.　It　is　constructed　with　negatively　charged　adenosine　5-triphosphate　(ATP)　and　negatively　charged　fluorescein　diphosphate　(FDP),　which　are　used　as　fluorescence　detection,　and　a　cationic　diblock　copolymer　methoxy-poly(ethylene　glycol)(113)-b-poly(2-dimethyl-aminoethyl　methacrylate)(70).　Upon　addition　of　calf　intestinal　alkaline　phosphatase,　the　superamphiphile　disintegrates,　presumably　due　to　the　enzymatic　hydrolysis　of　ATP.　This　process　is　accompanied　by　an　increase　in　the　fluorescence　emission　intensity　of　fluorescein　owing　to　the　hydrolysis　of　FDP.　The　in　vitro　application　of　the　superamphiphile　is　also　proven.　Thus,　the　turn-on　fluorescence　of　the　superamphiphile　serves　as　a　real-time　module　for　detection　of　the　disintegration　of　superamphiphile.