AIM:　To　investigate　the　associations　between　fruit　and　vegetable　consumption　and　risk　of　asthenopia　among　Chinese　college　students.　METHODS:　A　total　of　1022　students　were　selected　from　five　universities　by　a　multi-stage　stratified　cluster　sampling　method.　They　were　surveyed　via　a　self-administered　questionnaire　including　socio-demographic　features,　dietary　and　lifestyle　habits,　eye-related　symptoms,　eye　care　habits　and　history　of　diseases.　Ascertainment　of　asthenopia　was　based　on　participants＇　subjectively　reported　symptoms.　The　associations　between　fruit　and　vegetable　intake　with　asthenopia　risk　were　assessed　using　multivariate　logistic　regression　analysis.　RESULTS:　There　were　no　significant　associations　between　total　fruit　and　vegetable,　total　vegetable,　or　fruit　and　the　risk　of　asthenopia.　Higher　intake　of　dark-green　leafy　vegetable　was　likely　to　be　inversely　associated　with　asthenopia　risk　[　odd　ratio　(OR):　0.60;　95%　CI:　0.37-0.97;　(Ptrend)=0.21]　after　controlling　for　nondietary　and　dietary　risk　factors.　Stratified　analysis　showed　that　the　inverse　association　between　dark-green　leafy　vegetable　intake　and　asthenopia　risk　was　limited　to　participants　with　suboptimal　eyesight　(OR:　0.45;　95%　CI:　0.25-0.82;　P-trend=　0.05),　wearing　glasses　(OR:　0.35;　95%　CI:　0.17-0.72;　P-trend=　0.03)　or　using　computer　＞=　3h/d　(OR:　0.48;　95%　CI:　0.25-0.93;　P-trend=　0.08).　CONCLUSION:　A　higher　consumption　of　dark-green　leafy　vegetable　is　associated　with　a　lower　asthenopia　risk　among　college　students　with　suboptimal　eyesight　and　poor　eye　care　habits.