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Abstract:
Bromelain, a cysteine endopeptidase enzyme of great commercial value, has been widely used in food, pharmaceutical, and cosmetic industries. Conventional methods for purification of bromelain are still limited by a low binding efficiency, time-consuming process, and expensive equipment. Therefore, for selective absorption of bromelain, we developed a facile and effective method to fabricate magnetic mesoporous molecularly imprinted polymers using pericarpium granati-derived carbon as the carrier for the first time. The characterizations of the imprinted polymers indicated that a polydopamine layer was coated on the surface of the carrier and the crystallinity of the carrier did not change. The obtained imprinted polymers exhibited favourable saturation magnetization, a high adsorption capacity of 135.96 mg g(-1), a fast equilibrium time, and satisfactory reusability. The imprinted polymers were prepared by an eco-friendly method and exhibited rapid separation and good adsorption performance, thus making the method applicable to biomacromolecular separation, proteomic analysis, and biomedical research.
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FOOD CHEMISTRY
ISSN: 0308-8146
Year: 2018
Volume: 256
Page: 91-97
5 . 3 9 9
JCR@2018
7 . 5 1 4
JCR@2020
ESI Discipline: AGRICULTURAL SCIENCES;
ESI HC Threshold:87
JCR Journal Grade:2
CAS Journal Grade:1
Cited Count:
WoS CC Cited Count: 29
SCOPUS Cited Count: 32
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 6