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学者姓名:王嗣岑

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< Page ,Total 17 >
Inkjet-Based Dispersive Liquid-Liquid Microextraction Method Coupled with UHPLC-MS/MS for the Determination of Aflatoxins in Wheat. PubMed SCIE
期刊论文 | 2019 | Analytical chemistry
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Abstract :

A novel method was developed for determination of aflatoxin B1, B2, G1, and G2 (AFB1, AFB2, AFG1, and AFG2) in wheat using inkjet-based dispersive liquid-liquid microextraction (DLLME) coupled with ultrahigh-pressure liquid chromatography-tandem mass spectrometry. A drop-on-demand jetting device was used to form a cloudy solution in traditional DLLME by injecting extraction solvent (10 μL) as ultrafine droplets (∼20 μm diameter) at high frequency into sample solution. The method was validated using wheat as a representative matrix, which was pretreated with acetonitrile/water solution. Good linearity was observed over the studied range (0.06-6 μg/kg), and the limits of quantification (0.06-0.18 μg/kg) were below the maximum level established by the European Union for cereal. Satisfactory recoveries, ranging from 83.2% to 93.0% with relative standard deviations below 4.6%, were obtained for all compounds. The method, which is convenient and reliable and has low solvent consumption, represents a new direction for the development of traditional DLLME technology.

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GB/T 7714 Sun Meng , Xu Dan , Wang Sicen et al. Inkjet-Based Dispersive Liquid-Liquid Microextraction Method Coupled with UHPLC-MS/MS for the Determination of Aflatoxins in Wheat. [J]. | Analytical chemistry , 2019 .
MLA Sun Meng et al. "Inkjet-Based Dispersive Liquid-Liquid Microextraction Method Coupled with UHPLC-MS/MS for the Determination of Aflatoxins in Wheat." . | Analytical chemistry (2019) .
APA Sun Meng , Xu Dan , Wang Sicen , Uchiyama Katsumi . Inkjet-Based Dispersive Liquid-Liquid Microextraction Method Coupled with UHPLC-MS/MS for the Determination of Aflatoxins in Wheat. . | Analytical chemistry , 2019 .
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A thiopyran derivative with low murine toxicity with therapeutic potential on lung cancer acting through a NF-κB mediated apoptosis-to-pyroptosis switch. PubMed SCIE
期刊论文 | 2019 , 24 (1-2) , 74-82 | Apoptosis : an international journal on programmed cell death
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Abstract :

Pyroptosis is a novel manner of cell death that can be mediated by chemotherapy drugs. The awareness of pyroptosis is significantly increasing in the fields of anti-tumor research and chemotherapy drugs. Invoking the occurrence of pyroptosis is an attractive prospect for the treatment of lung cancer. Here, the compound L61H10 was obtained as a thiopyran derivative to compare its activity with curcumin. It was indicated that L61H10 exhibited good anti-tumor activity both in vitro and in vivo via the switch of apoptosis-to-pyroptosis, which was associated with the NF-κB signaling pathway. In addition, L61H10 had no obvious side effects both in vitro and in vivo. In brief, L61H10 is shown to be a potential anti-lung cancer agent and research on its anti-tumor mechanism provides new information for chemotherapy drug research.

Keyword :

Pyroptosis Apoptosis Toxicity Anti-lung cancer NF-κB

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GB/T 7714 Chen Liping , Weng Bixia , Li Huimin et al. A thiopyran derivative with low murine toxicity with therapeutic potential on lung cancer acting through a NF-κB mediated apoptosis-to-pyroptosis switch. [J]. | Apoptosis : an international journal on programmed cell death , 2019 , 24 (1-2) : 74-82 .
MLA Chen Liping et al. "A thiopyran derivative with low murine toxicity with therapeutic potential on lung cancer acting through a NF-κB mediated apoptosis-to-pyroptosis switch." . | Apoptosis : an international journal on programmed cell death 24 . 1-2 (2019) : 74-82 .
APA Chen Liping , Weng Bixia , Li Huimin , Wang Haonan , Li Qian , Wei Xiaoyan et al. A thiopyran derivative with low murine toxicity with therapeutic potential on lung cancer acting through a NF-κB mediated apoptosis-to-pyroptosis switch. . | Apoptosis : an international journal on programmed cell death , 2019 , 24 (1-2) , 74-82 .
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Magnetic carbon nanotubes camouflaged with cell membrane as a drug discovery platform for selective extraction of bioactive compounds from natural products SCIE
期刊论文 | 2019 , 364 , 269-279 | CHEMICAL ENGINEERING JOURNAL
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The biomimetic functionalization of carbon nanotubes (CNTs) are promising for its application in solid phase extraction because they can overcome current limitations of covalent functionalization and obtain desired specific recognition ability. The present study aimed to develop a novel dual functionalization CNTs modified with magnetic nanoparticles and high alpha(1A)-adrenergic receptor (alpha(1A)-AR) expression HEK 293 cell membrane. On the basis of the specific interactions between drugs and receptors on cell membrane, high alpha(1A)-AR expression cell membrane camouflaged magnetic CNTs (CMMCNTs) were employed as a drug discovery platform to screen potential alpha(1A)-AR antagonists from traditional Chinese medicine (TCM). The resultant CMMCNTs had a right-side-out membrane and desired magnetic property, enabling to rapidly target and separate the bioactive compounds from complex samples. Furthermore, the CMMCNTs possessed high adsorption capacity, fast binding kinetics, and satisfactory selectivity. As a result, two bioactive compounds as lappaconitine and benzoylmesaconine were screened and identified in the actual application. Moreover, the lappaconitine was validated to have favorable antagonism to alpha(1A)-AR through preliminary pharmacological assays. Hence, this successful application of CMMCNTs showed significant advantages in drug discovery so as to drive the development of TCM. More importantly, the cell membrane biomimetic modification could be widely used to improve the performance of CNTs in various applications.

Keyword :

Cell membrane-biomimetic functionalization Drug discovery Magnetic carbon nanotubes TCM bioactive compounds screening Receptor-ligand interaction

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GB/T 7714 Hu, Qi , Bu, Yusi , Zhen, Xueyan et al. Magnetic carbon nanotubes camouflaged with cell membrane as a drug discovery platform for selective extraction of bioactive compounds from natural products [J]. | CHEMICAL ENGINEERING JOURNAL , 2019 , 364 : 269-279 .
MLA Hu, Qi et al. "Magnetic carbon nanotubes camouflaged with cell membrane as a drug discovery platform for selective extraction of bioactive compounds from natural products" . | CHEMICAL ENGINEERING JOURNAL 364 (2019) : 269-279 .
APA Hu, Qi , Bu, Yusi , Zhen, Xueyan , Xu, Ke , Ke, Ruifang , Xie, Xiaoyu et al. Magnetic carbon nanotubes camouflaged with cell membrane as a drug discovery platform for selective extraction of bioactive compounds from natural products . | CHEMICAL ENGINEERING JOURNAL , 2019 , 364 , 269-279 .
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Liquid chromatography coupled with hybrid ion trap time-of-flight mass spectrometry method for the determination of caulophine in rat bio-samples and its pharmacokinetic study after intragastric and intraperitoneal administration EI SCIE PubMed Scopus
期刊论文 | 2018 , 41 (2) , 422-431 | JOURNAL OF SEPARATION SCIENCE
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A rapid and precise liquid chromatography coupled with hybrid ion trap/time-of-flight mass spectrometry method to detect and quantify caulophine and its possible active metabolites in rat plasma and urine was developed. Samples were prepared by plasma protein precipitation combined with a liquid-liquid extraction method. The separation was carried out on an InertSustain (R) C18 column with a mobile phase comprising methanol and 0.1% aqueous formic acid solution. The analysis was complete in 20 min with a flow rate of 0.4 mL/min. Taspine was used as the internal standard. Mass spectrometric detection was conducted with hybrid ion trap/time-of-flight equipped with electrospray ionization in the positive ion mode. The calibration curves of caulophine were linear over the concentration ranges of 0.002-0.20 mu g/mL for plasma and 0.005-0.50 mu g/mL for urine with the correlation coefficients greater than 0.998 in both cases. The method was successfully used to investigate the pharmacokinetics and bioavailability in rat plasma and urine samples after intragastric and intraperitoneal administration of caulophine sodium salt.

Keyword :

pharmacokinetics caulophine metabolites mass spectrometry

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GB/T 7714 Lu, Dan , Xu, Xiao , Li, Chunlei et al. Liquid chromatography coupled with hybrid ion trap time-of-flight mass spectrometry method for the determination of caulophine in rat bio-samples and its pharmacokinetic study after intragastric and intraperitoneal administration [J]. | JOURNAL OF SEPARATION SCIENCE , 2018 , 41 (2) : 422-431 .
MLA Lu, Dan et al. "Liquid chromatography coupled with hybrid ion trap time-of-flight mass spectrometry method for the determination of caulophine in rat bio-samples and its pharmacokinetic study after intragastric and intraperitoneal administration" . | JOURNAL OF SEPARATION SCIENCE 41 . 2 (2018) : 422-431 .
APA Lu, Dan , Xu, Xiao , Li, Chunlei , Wang, Sicen . Liquid chromatography coupled with hybrid ion trap time-of-flight mass spectrometry method for the determination of caulophine in rat bio-samples and its pharmacokinetic study after intragastric and intraperitoneal administration . | JOURNAL OF SEPARATION SCIENCE , 2018 , 41 (2) , 422-431 .
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Improved cell membrane bioaffinity sample pretreatment technique with enhanced stability for screening of potential allergenic components from traditional Chinese medicine injections EI SCIE Scopus
期刊论文 | 2018 , 6 (4) , 624-633 | JOURNAL OF MATERIALS CHEMISTRY B
WoS CC Cited Count: 2 SCOPUS Cited Count: 3
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Aiming at improving reliability and tedious analysis time in conventional cell membrane chromatography, an improved bioaffinity sample pretreatment technique with enhanced stability was developed to fast screen and extract potential allergenic components from traditional Chinese medicine injections. In this study, rat basophilic leukemia-2H3 cell membrane coated silica particles (RBL-2H3/CMCSPs) were fabricated by irreversible adsorption between the cell membrane and silica and self-fusion of the cell membrane, which could simulate drug-receptor interactions in vitro. Also, benefiting from the use of paraformaldehyde, the average recoveries of the six batches of RBL/CMCSPs were 90.2% with a relative standard deviation of less than 7.8%, showing that the stability of the materials was remarkably improved compared to materials without fixation. After the successful characterization by spectroscopic and imaging instruments, the prepared RBL-2H3/CMCSPs exhibited desirable adsorption capacity and selectivity. The RBL-2H3/CMCSPs combined with high performance liquid chromatography coupled with time of flight mass spectrometry were then successfully applied to screen and identify two potential allergenic components from huangqi injection, the allergenic activities of which were further investigated by beta-hexosaminidase release assay and histamine release assay in vitro. Overall, this work provides a good platform for the fabrication of bioaffinity sample pretreatment materials with high stability and a long lifespan, which can be a time-saving and energy-saving bioaffinity method to rapidly screen and preconcentrate target compounds from complex samples.

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GB/T 7714 Bu, Yusi , Hu, Qi , Xu, Ke et al. Improved cell membrane bioaffinity sample pretreatment technique with enhanced stability for screening of potential allergenic components from traditional Chinese medicine injections [J]. | JOURNAL OF MATERIALS CHEMISTRY B , 2018 , 6 (4) : 624-633 .
MLA Bu, Yusi et al. "Improved cell membrane bioaffinity sample pretreatment technique with enhanced stability for screening of potential allergenic components from traditional Chinese medicine injections" . | JOURNAL OF MATERIALS CHEMISTRY B 6 . 4 (2018) : 624-633 .
APA Bu, Yusi , Hu, Qi , Xu, Ke , Xie, Xiaoyu , Wang, Sicen . Improved cell membrane bioaffinity sample pretreatment technique with enhanced stability for screening of potential allergenic components from traditional Chinese medicine injections . | JOURNAL OF MATERIALS CHEMISTRY B , 2018 , 6 (4) , 624-633 .
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The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR SCIE PubMed Scopus
期刊论文 | 2018 , 8 | SCIENTIFIC REPORTS
WoS CC Cited Count: 1
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Coptidis Rhizoma binds to the membrane receptors on hPDLSC/CMC, and the active ingredient Berberine (BER) that can be extracted from it may promote the proliferation and osteogenesis of periodontal ligament stem cells (hPDLSC). The membrane receptor that binds with BER on the cell surface of hPDLSC, the mechanism of direct interaction between BER and hPDLSC, and the related signal pathway are not yet clear. In this research, EGFR was screened as the affinity membrane receptor between BER and hPDLSC, through retention on CMC, competition with BER and by using a molecular docking simulation score. At the same time, the MAPK PCR Array was selected to screen the target genes that changed when hPDLSC was simulated by BER. In conclusion, BER may bind to EGFR on the cell membrane of hPDLSC so the intracellular ERK signalling pathways activate, and nuclear-related genes of FOS change, resulting in the effect of osteogenesis on PDLSC.

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GB/T 7714 Liu, Jin , Zhao, Xiaodan , Pei, Dandan et al. The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR [J]. | SCIENTIFIC REPORTS , 2018 , 8 .
MLA Liu, Jin et al. "The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR" . | SCIENTIFIC REPORTS 8 (2018) .
APA Liu, Jin , Zhao, Xiaodan , Pei, Dandan , Sun, Guo , Li, Ye , Zhu, Chunhui et al. The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR . | SCIENTIFIC REPORTS , 2018 , 8 .
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Methods to treat the industrial wastewater in diosgenin enterprises produced from Diosorea zingiberensis C. H. Wright EI SCIE Scopus
期刊论文 | 2018 , 186 , 34-44 | JOURNAL OF CLEANER PRODUCTION
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Diosgenin wastewater produced from Dioscorea zingiberensis C. H. Wright in diosgenin enterprise is the by-product of numerous operations chiefly including washing raw materials, acid hydrolysis, and cleaning the apparatus with water. It requires the removal of high concentrations of various organic and inorganic contaminants from this wastewater in order to avoid pollution to the environment and fresh water system. Currently, several different methods such as chemical, physicochemical, biological recovery resource, constructed wetland, and integrated methods of these processes are being applied for treating diosgenin wastewater. Aiming to get a comprehensive overview, available reports from experimental scale to industry scale about the treatment of this wastewater were collected and summarized. Therefore, this review paper will provide appropriate choice for the efficient treatment of diosgenin wastewater and supportive information for the other scientists who want to continue this valuable research in the future. (C) 2018 Elsevier Ltd. All rights reserved.

Keyword :

Chemical Biological Physicochemical Diosgenin wastewater Dioscorea zingiberensis C. H. Wright

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GB/T 7714 Zhang, Xinxin , Jin, Ming , Tadesse, Nigatu et al. Methods to treat the industrial wastewater in diosgenin enterprises produced from Diosorea zingiberensis C. H. Wright [J]. | JOURNAL OF CLEANER PRODUCTION , 2018 , 186 : 34-44 .
MLA Zhang, Xinxin et al. "Methods to treat the industrial wastewater in diosgenin enterprises produced from Diosorea zingiberensis C. H. Wright" . | JOURNAL OF CLEANER PRODUCTION 186 (2018) : 34-44 .
APA Zhang, Xinxin , Jin, Ming , Tadesse, Nigatu , Zhan, Guanqun , Zhang, Hui , Dang, Jun et al. Methods to treat the industrial wastewater in diosgenin enterprises produced from Diosorea zingiberensis C. H. Wright . | JOURNAL OF CLEANER PRODUCTION , 2018 , 186 , 34-44 .
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Screening of bioactive components from traditional Chinese medicines using cell membrane chromatography coupled with mass spectrometry SCIE PubMed Scopus
期刊论文 | 2018 , 29 (4) , 341-350 | PHYTOCHEMICAL ANALYSIS
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IntroductionCell membrane chromatography (CMC), as a highly selective type of affinity chromatography, has been demonstrated as an effective method to screen bioactive components acting on specific receptor from a complicated biological system. ObjectiveTo review the recent research progress and the technical applications of these analytical methods using CMC combined with gas chromatography-mass spectrometry, (GC/MS) and liquid chromatography-mass spectrometry (LC/MS). MethodologyIn this review, we briefly introduce the CMC offline GC/MS, CMC online GC/MS, CMC offline LC/MS, and CMC online LC/MS system. And the practical application of these technologies is also enumerated. Then the future of these technologies and research methods were discussed. ResultsMany bioactive components interacting with specific receptors have been screened and identified in traditional Chinese medicines. ConclusionCMC technique has been combined with GC/MS and HPLC/MS and these combined systems have been successfully used to screen bioactive components acting on specific receptors from a complicated biological system. We briefly introduce the CMC offline GC/MS, CMC online GC/MS, CMC offline LC/MS, and CMC online LC/MS system. And the practical application of these technologies is also enumerated. The CMC technique has been combined with GC/MS and HPLC/MS and these combined systems have been successfully used to screen bioactive components acting on specific receptors from a complicated biological system.

Keyword :

cell membrane chromatography liquid chromatography-mass spectrometry target components multi-dimensional chromatography gas chromatography-mass spectrometry

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GB/T 7714 Han, Shengli , Lv, Yanni , Wei, Fen et al. Screening of bioactive components from traditional Chinese medicines using cell membrane chromatography coupled with mass spectrometry [J]. | PHYTOCHEMICAL ANALYSIS , 2018 , 29 (4) : 341-350 .
MLA Han, Shengli et al. "Screening of bioactive components from traditional Chinese medicines using cell membrane chromatography coupled with mass spectrometry" . | PHYTOCHEMICAL ANALYSIS 29 . 4 (2018) : 341-350 .
APA Han, Shengli , Lv, Yanni , Wei, Fen , Fu, Jia , Hu, Qi , Wang, Sicen . Screening of bioactive components from traditional Chinese medicines using cell membrane chromatography coupled with mass spectrometry . | PHYTOCHEMICAL ANALYSIS , 2018 , 29 (4) , 341-350 .
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Stepwise frontal affinity chromatography model for drug and protein interaction EI SCIE PubMed Scopus
期刊论文 | 2018 , 410 (23) , 5807-5815 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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Frontal affinity chromatography is an efficient technique that combines affinity interaction and high-performance liquid chromatography, and frontal analysis has been used in studying the interaction between drugs and proteins. Based on frontal analysis, stepwise frontal analysis has been established. The present study aimed to use the Lineweaver-Burk plot in stepwise frontal analysis by taking the weighted average of time data. Commercial human serum albumin (HSA) and alpha-1-acid glycoprotein (AGP) columns were used as an affinity column. Warfarin and digitoxin were chosen as model drugs for the HSA column, whereas verapamil and tamsulosin were selected as model drugs for the AGP column. The time data obtained by frontal analysis and stepwise frontal analysiswere compared, and the results revealed good correlation (r2 = 0.9946-0.9998). Frontal analysis and stepwise frontal analysis were also used to analyze the equilibrium dissociation constants (Kd) of model drugs on the HSA and AGP columns. The Kd values were compared with literature values, which revealed the same order of magnitude. These results illustrate that conversion of the time data is reasonable and feasible. The Lineweaver-Burk plot can be used in the stepwise frontal analysis model to study the characteristics of the interaction between drugs and proteins.

Keyword :

Stepwise frontal analysis model Affinity chromatography Equilibrium dissociation constants (K-d) Lineweaver-Burk plot

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GB/T 7714 He, Xiaoshuang , Sui, Yue , Wang, Sicen . Stepwise frontal affinity chromatography model for drug and protein interaction [J]. | ANALYTICAL AND BIOANALYTICAL CHEMISTRY , 2018 , 410 (23) : 5807-5815 .
MLA He, Xiaoshuang et al. "Stepwise frontal affinity chromatography model for drug and protein interaction" . | ANALYTICAL AND BIOANALYTICAL CHEMISTRY 410 . 23 (2018) : 5807-5815 .
APA He, Xiaoshuang , Sui, Yue , Wang, Sicen . Stepwise frontal affinity chromatography model for drug and protein interaction . | ANALYTICAL AND BIOANALYTICAL CHEMISTRY , 2018 , 410 (23) , 5807-5815 .
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Dioscorea zingiberensis C. H. Wright: An overview on its traditional use, phytochemistry, pharmacology, clinical applications, quality control, and toxicity SCIE PubMed Scopus
期刊论文 | 2018 , 220 , 283-293 | JOURNAL OF ETHNOPHARMACOLOGY
SCOPUS Cited Count: 1
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Ethnopharmacological relevance: Dioscorea zingiberensis C. H. Wright (D. zingiberensis), Dioscoreaceae, is used extensively in traditional Chinese medicines. The aim of the current review paper is to give a comprehensive overview of the traditional usage and phytochemistry of the plant. Clinical studies performed and products prepared from the plant and active principles will be mentioned. In addition a review of the taxonomy of the genus Dioscorea is given. Materials and methods: A systematic search on literature has been performed in databases like Google Scholar, Science Direct, Scifinder, Web of Science, and in Chinese databases (China Knowledge Resource Integrated, i.e., CNKI and Wanfang) including Ph.D. and M.Sc. dissertations. These from the period 1983-2016 have been searched. Results: D. zingiberensis is an endemic plant in China widely distributed in some provinces, especially Hubei and Shaanxi. The rhizomes are the medicinal part in Dun-Ye-Guan-Xin-Ning tablets and are used for isolation of diosgenin. Steroidal saponins are believed to be the active principles. More than 70 compounds have been identified. Several of these have been tested in preclinical assays and clinical trials. A wide spectrum of biological effects including cardiovascular, anti-thrombosis, hyperlipidemia, neuroprotection, anti-inflammatory, and anthelmintic effect has been verified. Conclusions: Because of the promising results from the investigations on the plant material of D. zingiberensis, further in depth analyses ought to be performed to evaluate its potential as either a traditional drug or a source of bioactive principle. The presently performed studies do not explain mechanism of action, pharmacokinetics (ADME properties), or toxicity. All of these topics need more elaborate investigations.

Keyword :

Photochemistry Pharmacology Botanical profiles Quality control Dioscorea zingiberensis C. H. Wright Toxicity

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GB/T 7714 Zhang, Xinxin , Jin, Ming , Tadesse, Nigatu et al. Dioscorea zingiberensis C. H. Wright: An overview on its traditional use, phytochemistry, pharmacology, clinical applications, quality control, and toxicity [J]. | JOURNAL OF ETHNOPHARMACOLOGY , 2018 , 220 : 283-293 .
MLA Zhang, Xinxin et al. "Dioscorea zingiberensis C. H. Wright: An overview on its traditional use, phytochemistry, pharmacology, clinical applications, quality control, and toxicity" . | JOURNAL OF ETHNOPHARMACOLOGY 220 (2018) : 283-293 .
APA Zhang, Xinxin , Jin, Ming , Tadesse, Nigatu , Dang, Jun , Zhou, Tao , Zhang, Hui et al. Dioscorea zingiberensis C. H. Wright: An overview on its traditional use, phytochemistry, pharmacology, clinical applications, quality control, and toxicity . | JOURNAL OF ETHNOPHARMACOLOGY , 2018 , 220 , 283-293 .
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