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Nanoporous carbon spheres derived from metal-phenolic coordination polymers for supercapacitor and biosensor SCIE
期刊论文 | 2019 , 544 , 241-248 | JOURNAL OF COLLOID AND INTERFACE SCIENCE
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Abstract :

The conversion of plant polyphenol to multi-functional nanoporous carbon spheres (NCSs) is desirable and challenging. Tannic acid (TA), as one kind of plant polyphenol, has been regarded as one of potential renewable carbon sources for nanoporous carbon materials. However, the nanoporous carbon materials derived from TA usually show low surface area and irregular morphology, which partially limit their broad applications. Herein, we demonstrate the successful synthesis of NCSs with high surface area (up to 2221 m(2)/g) and uniform diameter (-120 nm) by direct carbonization of zinc-phenolic coordination polymer using TA as a carbon source. The zinc contents play an important role in tailoring the porosity of NCSs. The specific surface area of NCSs can be adjusted in the range of 527-2221 m(2)/g as the zinc contents in the coordination polymers changed from 0 to 8.6 wt%. To verify the multi-functions of NCSs, such carbon spheres are further used as a sensing platform for the analysis of nucleic acid variants with high selectivity and low limit of detection, and electrode material for supercapacitor with high specific capacitance, good capacitance retention, and excellent cyclic stability. (C) 2019 Elsevier Inc. All rights reserved.

Keyword :

Carbon Coordination polymer Sensor Supercapacitor Mesoporous material

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GB/T 7714 Wang, Gen , Qin, Jing , Zhao, Yongxi et al. Nanoporous carbon spheres derived from metal-phenolic coordination polymers for supercapacitor and biosensor [J]. | JOURNAL OF COLLOID AND INTERFACE SCIENCE , 2019 , 544 : 241-248 .
MLA Wang, Gen et al. "Nanoporous carbon spheres derived from metal-phenolic coordination polymers for supercapacitor and biosensor" . | JOURNAL OF COLLOID AND INTERFACE SCIENCE 544 (2019) : 241-248 .
APA Wang, Gen , Qin, Jing , Zhao, Yongxi , Wei, Jing . Nanoporous carbon spheres derived from metal-phenolic coordination polymers for supercapacitor and biosensor . | JOURNAL OF COLLOID AND INTERFACE SCIENCE , 2019 , 544 , 241-248 .
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Programming in situ accelerated DNA walkers in diffusion-limited microenvironments SCIE
期刊论文 | 2019 , 10 (10) , 3103-3109 | CHEMICAL SCIENCE
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Macromolecule diffusion in cellular microenvironments dictates the kinetics of biochemical processes, yet inevitably limiting the assembly and operation of biomimetic motors. Herein we program in situ accelerated DNA walkers in diffusion-limited microenvironments such as molecularly crowded solutions and cytoplasm. All DNA components, including single-foot walkers, chemically damaged tracks and calibration elements, are anchored on individual gold nanoparticles. Two endogenous enzymes participating in base repair pathways are used to actuate on-particle walking via a base excision/ hydrolyzation coupled reaction. The walkers are in situ driven without requiring external drivers and accelerated several times. They also avoid low-efficiency diffusion/ assembly procedures and respond to heterogeneous cellular milieus with calibration function. We further regulated the walking kinetics via DNA densities and sets of enzymes, and demonstrated cytoplasmic behaviors of three kinds of walkers. They were utilized to profile DNA repair pathways and monitor enzyme catalysis in living cells.

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GB/T 7714 Chen, Feng , Xue, Jing , Bai, Min et al. Programming in situ accelerated DNA walkers in diffusion-limited microenvironments [J]. | CHEMICAL SCIENCE , 2019 , 10 (10) : 3103-3109 .
MLA Chen, Feng et al. "Programming in situ accelerated DNA walkers in diffusion-limited microenvironments" . | CHEMICAL SCIENCE 10 . 10 (2019) : 3103-3109 .
APA Chen, Feng , Xue, Jing , Bai, Min , Qin, Jing , Zhao, Yongxi . Programming in situ accelerated DNA walkers in diffusion-limited microenvironments . | CHEMICAL SCIENCE , 2019 , 10 (10) , 3103-3109 .
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All-in-One Synchronized DNA Nanodevices Facilitating Multiplexed Cell Imaging SCIE
期刊论文 | 2019 , 91 (7) , 4696-4701 | ANALYTICAL CHEMISTRY
WoS CC Cited Count: 1
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Multifunctional DNA nanodevices perform ever more tasks with applications ranging from in vitro biomarker detection to in situ cell imaging. However, most developed ones consist of a series of split building blocks, which suffer from asynchronous behaviors in complicated cellular microenvironments (endocytosis pathway, diffusion-limited cytoplasm, etc.), causing the loss of stoichiometric information and additional postassembly processes. Herein, we constructed all-in-one DNA nanodevices to achieve synchronous multiplexed imaging. All DNA components, including two sets of probe modules (each containing target-specific walkers, i.e., hairpin tracks with chemically damaged bases), are modified on individual gold nanoparticles. This design not only enables their integrated internalization into cells, circumventing inhomogeneous distribution of different building blocks and increasing the local concentrations of the interacting modules, but also avoids the impact of stochastic diffusion in viscous cytoplasm. A couple of intracellular enzymes in situ actuate the synchronized motion of the modules, all on-particle, after specific recognition of intracellular targets (such as RNAs and proteins), thus facilitating synchronized, multiplexed cell imaging. Finally, the proposed all-in-one nanodevices were successfully applied to monitor intracellular microRNA-21 and telomerase expression levels. The flexible design can be extended to detect other cytoplasmic molecules and monitor related pathways by simply changing the sequences.

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GB/T 7714 Xue, Jing , Chen, Feng , Bai, Min et al. All-in-One Synchronized DNA Nanodevices Facilitating Multiplexed Cell Imaging [J]. | ANALYTICAL CHEMISTRY , 2019 , 91 (7) : 4696-4701 .
MLA Xue, Jing et al. "All-in-One Synchronized DNA Nanodevices Facilitating Multiplexed Cell Imaging" . | ANALYTICAL CHEMISTRY 91 . 7 (2019) : 4696-4701 .
APA Xue, Jing , Chen, Feng , Bai, Min , Can, Xiaowen , Huang, Ping , Zhao, Yongxi . All-in-One Synchronized DNA Nanodevices Facilitating Multiplexed Cell Imaging . | ANALYTICAL CHEMISTRY , 2019 , 91 (7) , 4696-4701 .
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A Forster Resonance Energy Transfer Ratiometric Probe Based on Quantum Dot-Cresyl Violet for Imaging Hydrogen Sulfide in Living Cells SCIE CSCD
期刊论文 | 2018 , 46 (1) , 39-47 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
WoS CC Cited Count: 1
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Hydrogen sulfide (H2S) has been confirmed as a significant endogenous gaseous signaling molecule involved in various physiological processes. To monitor H2S in living cells, a Forster resonance energy transfer ( FRET) ratiometric probe based on quantum dot. cresyl violet was developed. In this work, quantum dot nanospheres (QDS) were firstly synthesized via a facile ultrasonication emulsion strategy, and the mixture chloroform solution containing hydrophobic quantum dots and COOH-functionalized amphiphilic polymer were successfully transferred into the oil. in. water micelle. The negatively charged quantum dot nanospheres with quantum dots embedded in the polymer matrixes were successfully fabricated after the evaporation of chloroform. And then, these quantum dot nanospheres were condensed with positively charged cresyl violet. azide (CV-N-3) via electrostatic interaction to obtain the QDS-N-3 complexes. The as. prepared QDS-N-3 complexes were monodispersed nanospheres with an average diameter of about 120 nm. These complexes were taken up by the cell through endocytosis, and they were still stable even in wide pH range. In addition, the QDS-N-3 complexes exhibited no cellular toxicity which was verified by MTT assay. In this ratiometric probe, CV-N-3 as a FRET acceptor was conjugated to quantum dot nanospheres. The quantum dots emitted at 591 nm and served as the FRET donor; once the aryl azide on the CV-N-3 was reduced to aniline by H2S, the probe emitted at 620 nm. The ratiometric probe allowed the elimination of interference of excitation intensity, intracellular environment and other factors. Furthermore, this method also offered a general protocol for preparing nanosensors for monitoring various small molecular in living cells.

Keyword :

Cell imaging Cresyl violet Quantum dot Hydrogen sulfide Ratiometric fluorescence

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GB/T 7714 Bai Min , Cao Xiao-Wen , Chen Feng et al. A Forster Resonance Energy Transfer Ratiometric Probe Based on Quantum Dot-Cresyl Violet for Imaging Hydrogen Sulfide in Living Cells [J]. | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY , 2018 , 46 (1) : 39-47 .
MLA Bai Min et al. "A Forster Resonance Energy Transfer Ratiometric Probe Based on Quantum Dot-Cresyl Violet for Imaging Hydrogen Sulfide in Living Cells" . | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 46 . 1 (2018) : 39-47 .
APA Bai Min , Cao Xiao-Wen , Chen Feng , Zhao Yue , Zhao Yong-Xi . A Forster Resonance Energy Transfer Ratiometric Probe Based on Quantum Dot-Cresyl Violet for Imaging Hydrogen Sulfide in Living Cells . | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY , 2018 , 46 (1) , 39-47 .
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Polydopamine@Gold Nanowaxberry Enabling Improved SERS Sensing of Pesticides, Pollutants, and Explosives in Complex Samples EI SCIE PubMed Scopus
期刊论文 | 2018 , 90 (15) , 9048-9054 | ANALYTICAL CHEMISTRY
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Surface-enhanced Raman scattering (SERS) is a promising analysis technique for detecting various analytes in complex samples due to its unique vibrational fingerprints and high signal enhancement. However, impurity interference and substrate unreliability are direct suppression factors for practical application. Herein, we synthesize polydopamine@gold (PDA@Au) nanowaxberry, where Au nanoparticles are deposited on the surface of PDA sphere with high density and uniformity. Seed-mediated synthesis is used for fabrication of nanowaxberry. Au seeds are deposited on the surface of PDA sphere, then I ion coordinating ligand is employed to form stable AuI4- complex with AuCl4-, which decreases reduction potential of AuCl4- and avails formation of shell structure. Such nanowaxberry has high density of voids and gaps in three-dimensional space, which could absorb analytes and benefit practical SERS detection. Using malachite green as a model analyte, nanowaxberry realizes highly sensitive detection with low limit of detection (1 pM) and good reproducibility (relative standard deviation of about 10%). Meanwhile, the nanowaxberry is employed for practical detection of thiram, benzidine, and 2,4-dinitrotoluene in the environmental water, juice, apple peel, and soil. The high performance makes nanowaxberry to be potentially used for pesticides detection, pollutants monitoring, and forbidden explosives sensing in complex samples.

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GB/T 7714 Chen, Dongzhen , Zhu, Xiaodong , Huang, Jian et al. Polydopamine@Gold Nanowaxberry Enabling Improved SERS Sensing of Pesticides, Pollutants, and Explosives in Complex Samples [J]. | ANALYTICAL CHEMISTRY , 2018 , 90 (15) : 9048-9054 .
MLA Chen, Dongzhen et al. "Polydopamine@Gold Nanowaxberry Enabling Improved SERS Sensing of Pesticides, Pollutants, and Explosives in Complex Samples" . | ANALYTICAL CHEMISTRY 90 . 15 (2018) : 9048-9054 .
APA Chen, Dongzhen , Zhu, Xiaodong , Huang, Jian , Wang, Gen , Zhao, Yue , Chen, Feng et al. Polydopamine@Gold Nanowaxberry Enabling Improved SERS Sensing of Pesticides, Pollutants, and Explosives in Complex Samples . | ANALYTICAL CHEMISTRY , 2018 , 90 (15) , 9048-9054 .
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基于量子点-甲酚紫FRET比率探针的活细胞硫化氢成像 CSCD PKU
期刊论文 | 2018 , (1) , 39-47 | 分析化学
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Abstract :

硫化氢(H2S)作为一种重要的气体信号分子,其细胞水平异常与多种疾病的发生发展密切相关.本研究针对现存荧光探针的胞内非特异性响应问题,发展了一种基于量子点-甲酚紫的荧光能量共振转移(F?rster resonance energy transfer,FRET)探针,利用比率荧光分析,实现活细胞内H2S的灵敏成像.通过一步超声乳化法制备量子点纳米球,再结合叠氮甲酚紫(CV-N3)制得无机-有机杂化QDS-N3比率探针.此纳米探针尺寸均一,粒径约为120 nm,酸性稳定性高,且无细胞毒性.H2S可将CV-N3上的N3还原成NH2,生成的氨基甲酚紫(CV-NH2)作为FRET-受体,吸收量子点纳米球的橙色荧光,产生红光信号.通过两个波长的荧光强度的比率分析可有效消除光源强度波动和细胞内复杂环境等的干扰,最终实现了活细胞内H2S成像.

Keyword :

量子点 甲酚紫 细胞成像 硫化氢 比率荧光

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GB/T 7714 白敏 , 曹晓文 , 陈锋 et al. 基于量子点-甲酚紫FRET比率探针的活细胞硫化氢成像 [J]. | 分析化学 , 2018 , (1) : 39-47 .
MLA 白敏 et al. "基于量子点-甲酚紫FRET比率探针的活细胞硫化氢成像" . | 分析化学 1 (2018) : 39-47 .
APA 白敏 , 曹晓文 , 陈锋 , 赵越 , 赵永席 . 基于量子点-甲酚紫FRET比率探针的活细胞硫化氢成像 . | 分析化学 , 2018 , (1) , 39-47 .
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Synthesis of spiny metal-phenolic coordination crystals as a sensing platform for sequence-specific detection of nucleic acids EI SCIE
期刊论文 | 2018 , 20 (47) , 7626-7630 | CRYSTENGCOMM
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Spiny metal-phenolic coordination crystals are synthesized using a nontoxic plant polyphenol as a ligand. Due to the abundance of metal species and sp(2)-hybridized carbon atoms on their spiny surface, such coordination crystals can adsorb DNA probes and quench the fluorescence, resulting in a high efficiency for nucleic acid assay.

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GB/T 7714 Huang, Hengbo , Qin, Jing , Wang, Gen et al. Synthesis of spiny metal-phenolic coordination crystals as a sensing platform for sequence-specific detection of nucleic acids [J]. | CRYSTENGCOMM , 2018 , 20 (47) : 7626-7630 .
MLA Huang, Hengbo et al. "Synthesis of spiny metal-phenolic coordination crystals as a sensing platform for sequence-specific detection of nucleic acids" . | CRYSTENGCOMM 20 . 47 (2018) : 7626-7630 .
APA Huang, Hengbo , Qin, Jing , Wang, Gen , Guo, Zehua , Yu, Xu , Zhao, Yongxi et al. Synthesis of spiny metal-phenolic coordination crystals as a sensing platform for sequence-specific detection of nucleic acids . | CRYSTENGCOMM , 2018 , 20 (47) , 7626-7630 .
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Self-Template Synthesis of Mesoporous Metal Oxide Spheres with Metal-Mediated Inner Architectures and Superior Sensing Performance EI Scopus SCIE
期刊论文 | 2018 , 28 (51) | Advanced Functional Materials
WoS CC Cited Count: 3 SCOPUS Cited Count: 2
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The development of a general strategy to synthesize mesoporous metal oxide spheres (MMOSs) with tailorable compositions and architectures is desirable and challenging. Herein, a general self-template strategy is demonstrated for the synthesis of MMOSs with tunable compositions (i.e., ZnO, Al2O3, Co3O4, Fe2O3, CuO) and metal-mediated inner architectures via thermal decomposition of metal-phenolic coordination polymers (MPCPs). The metal species in MPCPs can obviously influence the decomposition temperature of the polymer networks (Td). The inner architectures of MMOSs are determined by Td and the crystallization temperature of metal oxides (Tc). The MMOSs show solid inner architectures when Td > Tc or Td < Tc and hollow structure when Td ≈ Tc. Encouraged by their high surface area and accessible mesopores, gas sensors based on MMOSs (i.e., ZnO) are fabricated, which shows a low working temperature (250 °C) for detection of ethanol gas. The MMOSs (i.e., Co3O4) can further be used to fabricate sensing platform for detecting DNA analogue of miRNA-21 (a biomarker abnormally expressed in most of solid tumors). Such MMOSs show high sensitivity (0.19 × 10−9m) and can even efficiently distinguish the target DNA from single-, double- and triple-base mismatched DNA. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Keyword :

Coordination Polymers Crystallization temperature Decomposition temperature Mesoporous metal oxides Metal oxides Sensing performance Template synthesis Working temperatures

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GB/T 7714 Wang, Gen , Qin, Jing , Zhou, Xinran et al. Self-Template Synthesis of Mesoporous Metal Oxide Spheres with Metal-Mediated Inner Architectures and Superior Sensing Performance [J]. | Advanced Functional Materials , 2018 , 28 (51) .
MLA Wang, Gen et al. "Self-Template Synthesis of Mesoporous Metal Oxide Spheres with Metal-Mediated Inner Architectures and Superior Sensing Performance" . | Advanced Functional Materials 28 . 51 (2018) .
APA Wang, Gen , Qin, Jing , Zhou, Xinran , Deng, Yonghui , Wang, Huanting , Zhao, Yongxi et al. Self-Template Synthesis of Mesoporous Metal Oxide Spheres with Metal-Mediated Inner Architectures and Superior Sensing Performance . | Advanced Functional Materials , 2018 , 28 (51) .
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Functional and Biomimetic DNA Nanostructures on Lipid Membranes. EI PubMed SCIE
期刊论文 | 2018 , 34 (49) , 14721-14730 | Langmuir : the ACS journal of surfaces and colloids
WoS CC Cited Count: 3
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Sophisticated and dynamic membrane-anchored DNA nanostructures were developed to mimic a variety of membrane proteins, which play crucial roles in cellular functions. DNA biomimetic constructions bound on membranes are capable of modulating the morphologies, physical properties, and functions of lipid membranes, via mobility on membranes and/or inherent architectural features. This inspired young field of DNA-lipid-based nanobiomimetic systems is on the foundation of DNA nanotechnology. In this review, we highlight key successes in the development of structural DNA nanotechnology and demonstrate some typical static and dynamic complex DNA nanostructures first. Then, we discuss the biophysical properties of lipid membranes. Primary approaches are shown to attach hydrophilic DNA to hydrophobic lipid membranes. With appropriate designs, membrane-floating DNA nanostructures assemble and disassemble on membranes, modulated by  external stimuluses. The aggregation of DNA nanostructures could influence the physical properties of lipid membranes. We also summarize artificial nanochannels made of DNA, analogous to transmembrane proteins. Transformations of DNA nanopores might be achieved under certain conditions and realize the transport of small molecules across membranes. Next, we focus on membrane-shaping functions of membrane-anchored DNA nanostructures. Curvature of the membrane is closely related to the rich diversity of cellular functions. Mimicking membrane-sculpting proteins, such as BAR family domains and SNARE proteins etc., DNA biomimetic nanostructures induce the transformations of lipid membranes and modulate membrane adhesion and membrane fusion processes. Although recent studies in DNA nanostructure-lipid membrane biomimetic nanosystems have made great progress, this field is still facing many challenges. In the future, the designs of more elaborated DNA architectures will be explored. Sophisticated dynamic DNA nanostructures inspired by natural membrane machines will be driven by the synergistic effect of multiple interactions, including hydrophobic force, electrostatic force, and ligand-receptor interactions by chemical modifications on bases, to expand their applications in vivo from model membrane to cell membrane to karyotheca.

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GB/T 7714 Wu Na , Chen Feng , Zhao Yue et al. Functional and Biomimetic DNA Nanostructures on Lipid Membranes. [J]. | Langmuir : the ACS journal of surfaces and colloids , 2018 , 34 (49) : 14721-14730 .
MLA Wu Na et al. "Functional and Biomimetic DNA Nanostructures on Lipid Membranes." . | Langmuir : the ACS journal of surfaces and colloids 34 . 49 (2018) : 14721-14730 .
APA Wu Na , Chen Feng , Zhao Yue , Yu Xu , Wei Jing , Zhao Yongxi . Functional and Biomimetic DNA Nanostructures on Lipid Membranes. . | Langmuir : the ACS journal of surfaces and colloids , 2018 , 34 (49) , 14721-14730 .
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Engineered Janus probes modulate nucleic acid amplification to expand the dynamic range for direct detection of viral genomes in one microliter crude serum samples EI SCIE PubMed Scopus
期刊论文 | 2018 , 9 (2) , 392-397 | CHEMICAL SCIENCE
WoS CC Cited Count: 5 SCOPUS Cited Count: 5
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The viral genome load in diverse clinical samples varies over several orders of magnitude (e.g. 1-10(4) copies per mu L), thus a dynamic range-extended and sensitive analysis method is highly desired. However, existing well-developed nucleic acid amplification systems always suffer from either a limited dynamic range or modest sensitivity for analysis of these samples. Herein, we propose a general engineered Janus probe to modulate the thermodynamics and kinetic properties of the amplification reaction. Through rational regulation, the Janus system improves the performance by both reducing the background and enhancing the signal, expanding the operative dynamic range by 2 orders of magnitude. This proposed approach achieves a detection limit for hepatitis B virus (HBV) DNA of down to 3 copies and can be successfully applied for direct quantification of the HBV genome in one microliter crude serum samples without any pretreatment. The results are consistent with clinical diagnosis and hold considerable potential to discriminate healthy volunteers and patients at different disease stages. Whereas, following the same operation, the representative well-developed system provided serious false-negative results using such trace amounts of samples from clinically confirmed positive patients.

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GB/T 7714 Zhao, Yue , Chen, Feng , Qin, Jing et al. Engineered Janus probes modulate nucleic acid amplification to expand the dynamic range for direct detection of viral genomes in one microliter crude serum samples [J]. | CHEMICAL SCIENCE , 2018 , 9 (2) : 392-397 .
MLA Zhao, Yue et al. "Engineered Janus probes modulate nucleic acid amplification to expand the dynamic range for direct detection of viral genomes in one microliter crude serum samples" . | CHEMICAL SCIENCE 9 . 2 (2018) : 392-397 .
APA Zhao, Yue , Chen, Feng , Qin, Jing , Wei, Jing , Wu, Wenhua , Zhao, Yongxi . Engineered Janus probes modulate nucleic acid amplification to expand the dynamic range for direct detection of viral genomes in one microliter crude serum samples . | CHEMICAL SCIENCE , 2018 , 9 (2) , 392-397 .
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