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学者姓名：贺健康
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Abstract ：
Electrohydrodynamic (EHD) printing has been considered as a mature strategy to mimic the hierarchical microarchitectures in native extracellular matrix (ECM). Most of the EHD-printed scaffolds possess single-dimensional fibrous structures, which cannot mimic the multi-dimensional architectures for enhanced cellular behaviors. Here we developed a two-nozzle EHD printing system to fabricate hybrid scaffolds involving submicron and microscale features. The polyethylene oxide- polycaprolactone (PEO-PCL) submicron fibers were fabricated via solution-based EHD printing with a width of 527 +/- 56 nm. The PCL microscale fibers were fabricated via melt-based EHD printing with a width of 11.2 +/- 2.3 mu m. The hybrid scaffolds were fabricated by printing the submicron and microscale fibers in a layer-by-layer manner. The microscale scaffolds were utilized as a control group. Rat myocardial cells (H9C2 cells) were cultured on the two kinds of scaffolds for the culturing period of 1, 3 and 5 d. Biological results indicated that H9C2 cells showed enhanced adhesion and proliferation behaviors on the hybrid scaffold than those on the pure microscale scaffold. This work offers a facile and scalable strategy to fabricate multiscale synthetic scaffolds, which might be further explored to regulate cellular behaviors in the fields of tissue regeneration and biomedical engineering.
Keyword ：
cell proliferation electrohydrodynamic printing multiscale architecture synthetic scaffold
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| GB/T 7714 | Zhang, Bing , Li, Shikang , He, Jiankang et al. Electrohydrodynamic printing of submicron-microscale hybrid scaffolds with improved cellular adhesion and proliferation behaviors [J]. | NANOTECHNOLOGY , 2023 , 34 (10) . |
| MLA | Zhang, Bing et al. "Electrohydrodynamic printing of submicron-microscale hybrid scaffolds with improved cellular adhesion and proliferation behaviors" . | NANOTECHNOLOGY 34 . 10 (2023) . |
| APA | Zhang, Bing , Li, Shikang , He, Jiankang , Lei, Qi , Wu, Chuang , Song, Aiping et al. Electrohydrodynamic printing of submicron-microscale hybrid scaffolds with improved cellular adhesion and proliferation behaviors . | NANOTECHNOLOGY , 2023 , 34 (10) . |
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Abstract ：
Bioprinting has been widely investigated for tissue engineering and regenerative medicine applications. However, it is still difficult to reconstruct the complex native cell arrangement due to the limited printing resolution of conventional bioprinting techniques such as extrusion- and inkjet-based printing. Recently, an electrohydrodynamic (EHD) bioprinting strategy was reported for the precise deposition of well-organized cell-laden constructs with microscale filament size, whereas few studies have been devoted to developing bioinks that can be applied for EHD bioprinting and simultaneously support cell spreading. This study describes functionalized alginate-based bioinks for microscale EHD bioprinting using peptide grafting and fibrin incorporation, which leads to high cell viability (> 90%) and cell spreading. The printed filaments can be further refined to as small as 30 mu m by incorporating polyoxyethylene and remained stable over one week when exposed to an aqueous environment. By utilizing the presented alginate-based bioinks, layer-specific cell alignment along the printing struts could be observed inside the EHD-printed microscale filaments, which allows fabricating living constructs with cell-scale filament resolution for guided cellular orientation.
Keyword ：
Alginate-based bioinks Cell alignment Cell spreading Microscale electrohydrodynamic bioprinting
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| GB/T 7714 | Qiu, Zhennan , Zhu, Hui , Wang, Yutao et al. Functionalized alginate-based bioinks for microscale electrohydrodynamic bioprinting of living tissue constructs with improved cellular spreading and alignment [J]. | BIO-DESIGN AND MANUFACTURING , 2022 . |
| MLA | Qiu, Zhennan et al. "Functionalized alginate-based bioinks for microscale electrohydrodynamic bioprinting of living tissue constructs with improved cellular spreading and alignment" . | BIO-DESIGN AND MANUFACTURING (2022) . |
| APA | Qiu, Zhennan , Zhu, Hui , Wang, Yutao , Kasimu, Ayiguli , Li, Dichen , He, Jiankang . Functionalized alginate-based bioinks for microscale electrohydrodynamic bioprinting of living tissue constructs with improved cellular spreading and alignment . | BIO-DESIGN AND MANUFACTURING , 2022 . |
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Abstract ：
Severe tracheomalacia (TM) patients with respiratory symptoms need surgical intervention, including aortopexy, internal stents or external splint. While some patients continue to have respiratory symptoms after tracheal relief, and there is no evidence to support any one surgery therapy over another. Here we introduce a clinical safety and efficacy of the three-dimensional (3D)-printed bioresorbable airway external splints in treating congenital heart disease (CHD) patients with severe TM. From May 2019 to September 2020, nine patients with severe TM were enrolled. The median age was 5 months (range, 3-25 months), and the median weight was 7.5 kg (range, 3-15 kg). All patients had wheezing, and two patients were assisted by machine ventilation (MV) preoperatively. The median length of TM was 1.5 cm (range, 1.0-3.0 cm). All patients underwent suspension of a "C"-shaped lumen airway external splint, which were designed in SOLIDWORKS and made of polycaprolactone (PCL). The airway external splint could provided effective support for at least 6 months and was completely degraded into carbon dioxide and water within 2-3 years. The median time of postoperative machine assisted ventilation was 23.7 h (range, 3.3-223.4 h), and the median time of ICU stay was 9 days (range, 4-25 days). The median follow-up time was 18 months (range, 12-24 months). Respiratory symptoms were all relieved, and no external splint-associated complications occurred. The 3D computed tomography reconstruction showed no airway stenosis. Personalized 3D-printed bioresorbable airway external splint can not only limit external compression and prevent airway collapse but also ensure the growth potential of the airway, which is a safe, reliable and effective treatment for CHD with TM.
Keyword ：
congenital heart follow-up splint three-dimensional printing tracheomalacia
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| GB/T 7714 | Yu, Di , Peng, Wei , Mo, Xuming et al. Personalized 3D-Printed Bioresorbable Airway External Splint for Tracheomalacia Combined With Congenital Heart Disease [J]. | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY , 2022 , 10 . |
| MLA | Yu, Di et al. "Personalized 3D-Printed Bioresorbable Airway External Splint for Tracheomalacia Combined With Congenital Heart Disease" . | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 10 (2022) . |
| APA | Yu, Di , Peng, Wei , Mo, Xuming , Zhang, Yuxi , Zhang, Xing , He, Jiankang . Personalized 3D-Printed Bioresorbable Airway External Splint for Tracheomalacia Combined With Congenital Heart Disease . | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY , 2022 , 10 . |
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Abstract ：
Conductive hydrogels (CHs) have shown promising potential applied as wearable or epidermal sensors owing to their mechanical adaptability and similarity to natural tissues. However, it remains a great challenge to develop an integrated hydrogel combining outstanding conductive, self-healing and biocompatible performances with simple approaches. In this work, we propose a "one-pot" strategy to synthesize multifunctional CHs by incorporating two-dimensional (2D) transition metal carbides/nitrides (MXenes) multi-layer nano-flakes as nanofillers into oxidized alginate and gelatin hydrogels to form the composite CHs with various MXene contents. The presence of MXene with abundant surface groups and outstanding conductivity could improve the mechanical property and electroactivity of the composite hydrogels compared to pure oxidized alginate dialdehyde-gelatin (ADA-GEL). MXene-ADA-GELs kept good self-healing properties due to the dynamic imine linkage of the ADA-GEL network and have a promoting effect on mouse fibroblast (NH3T3s) attachment and spreading, which could be a result of the integration of MXenes with stimulating conductivity and hydrophily surface. This study suggests that the electroactive MXene-ADA-GELs can serve as an appealing candidate for skin wound healing and flexible bio-electronics.
Keyword ：
electrically conductive hydrogel MXene oxidized alginate self-healing wound healing
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| GB/T 7714 | Zhu, Hui , Dai, Weitao , Wang, Liming et al. Electroactive Oxidized Alginate/Gelatin/MXene (Ti3C2Tx) Composite Hydrogel with Improved Biocompatibility and Self-Healing Property [J]. | POLYMERS , 2022 , 14 (18) . |
| MLA | Zhu, Hui et al. "Electroactive Oxidized Alginate/Gelatin/MXene (Ti3C2Tx) Composite Hydrogel with Improved Biocompatibility and Self-Healing Property" . | POLYMERS 14 . 18 (2022) . |
| APA | Zhu, Hui , Dai, Weitao , Wang, Liming , Yao, Cong , Wang, Chenxi , Gu, Bingsong et al. Electroactive Oxidized Alginate/Gelatin/MXene (Ti3C2Tx) Composite Hydrogel with Improved Biocompatibility and Self-Healing Property . | POLYMERS , 2022 , 14 (18) . |
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Abstract ：
Here we developed a novel strategy to predict and control the electrothermal property and deformation pattern of electrically stimuli-responsive structures by printing programmable conductive patterns inside liquid crystal elastomer (LCE). It was found that the printed conductive patterns had excellent electrothermal performance and can be heated up to 120 degrees C within 12 s under the stimulus of an applied voltage. By controlling the width and spacing of the conductive lines, the electrothermal temperature of bilayer LCE structures can be regionally modulated, which subsequently determines the structural deformation for desired actuation. A finite element simulation method was established to accurately predict the effect of different conductive pattern design on the final deformation profiles, which showed a good consistence to the experimental results. The presented strategy exhibited unique capability in fabricating conductive pattern-embedded electrothermal structures for various programmable deformations like wing flapping, soft robot crawling and finger bending.
Keyword ：
3D printing deformation prediction electrothermal actuation liquid crystal elastomer (LCE) Programmable conductive pattern
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| GB/T 7714 | Huo, Ziyao , He, Jiankang , Pu, Huayan et al. Design and printing of embedded conductive patterns in liquid crystal elastomer for programmable electrothermal actuation [J]. | VIRTUAL AND PHYSICAL PROTOTYPING , 2022 , 17 (4) : 881-893 . |
| MLA | Huo, Ziyao et al. "Design and printing of embedded conductive patterns in liquid crystal elastomer for programmable electrothermal actuation" . | VIRTUAL AND PHYSICAL PROTOTYPING 17 . 4 (2022) : 881-893 . |
| APA | Huo, Ziyao , He, Jiankang , Pu, Huayan , Luo, Jun , Li, Dichen . Design and printing of embedded conductive patterns in liquid crystal elastomer for programmable electrothermal actuation . | VIRTUAL AND PHYSICAL PROTOTYPING , 2022 , 17 (4) , 881-893 . |
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Abstract ：
Leaf venation provides a promising template for engineering capillary-like vasculature in vitro due to its highly efficient fluid transport capability and remarkable similarities to native capillary networks. A key challenge in exploring the potential biological applications of leaf-inspired capillary networks (LICNs) is to accurately and quantitively understand its internal fluid transport characteristics. Here, a centerline-induced partition-assembly modeling strategy is proposed to establish a 3D computational model, which can accurately simulate the flow conditions in LICNs. Based on the 3D flow simulation, the authors demonstrate the excellent defect-resistant fluid transport capability of LICNs. Interestingly, structural defects in the primary channel can effectively accelerate the overall perfusion efficiency. Flow patterns in LICNs with multiple defects can be estimated by simple superposition of the simulation results derived from the corresponding single-defect models. The 3D computational model is further used to determine the optimal perfusion parameter for the in-vitro formation of endothelialized capillary networks by mimicking native microvascular flow conditions. The endothelialized networks can recapitulate the vascular colonization process and reveal a strong correlation between cancer cell adhesion and flow-induced shear stress. This study offers a quantitative tool to scrutinize the fluid and biological transport mechanisms within LICNs for various biomedical applications.
Keyword ：
fluid dynamics leaf-inspired capillary networks organ-on-a-chip
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| GB/T 7714 | Mao, Mao , Chen, Pengyu , He, Jiankang et al. Deciphering Fluid Transport Within Leaf-Inspired Capillary Networks Based on a 3D Computational Model [J]. | SMALL , 2022 , 18 (16) . |
| MLA | Mao, Mao et al. "Deciphering Fluid Transport Within Leaf-Inspired Capillary Networks Based on a 3D Computational Model" . | SMALL 18 . 16 (2022) . |
| APA | Mao, Mao , Chen, Pengyu , He, Jiankang , Zhu, Guangyu , Li, Xiao , Li, Dichen . Deciphering Fluid Transport Within Leaf-Inspired Capillary Networks Based on a 3D Computational Model . | SMALL , 2022 , 18 (16) . |
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Abstract ：
Micro/sub-microscale fibrillar architectures of extracellular matrix play important roles in regulating cellular behaviors such as attachment, migration, and differentiation. However, the interactions between cells and organized micro/ sub-microscale fibers have not been fully clarified yet. Here, the responses of MC3T3-E1 cells to electrohydrodynamic (EHD) printed scaffolds with microscale and/or sub-microscale fibrillar architectures were investigated to demonstrate their potential for bone tissue regeneration. Fibrillar scaffolds were EHD-fabricated with microscale (20.51 ?? 1.70 ??m) and/or sub-microscale (0.58 ?? 0.51 ??m) fibers in a controlled manner. The in vitro results showed that cells exhibited a 1.25-fold increase in initial attached cell number and 1.17-fold increase in vinculin expression on scaffolds with micro/sub-microscale fibers than that on scaffolds with pure microscale fibers. After 14 days of culture, the cells expressed 1.23 folds increase in collagen type I (COL-I) deposition compared with that on scaffolds with pure microscale fibers. These findings indicated that the EHD printed sub-microscale fibrous architectures can facilitate attachment and COL I secretion of MC3T3-E1 cells, which may provide a new insight to the design and fabrication of fibrous scaffolds for bone tissue engineering.
Keyword ：
Bone tissue engineering Cell-scaffold interaction Electrohydrodynamic printing Micro sub-microscale fibrous architectures
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| GB/T 7714 | Hu, Shugang , Meng, Zijie , Zhou, Junpeng et al. Enhanced Attachment and Collagen Type I Deposition of MC3T3-E1 Cells via Electrohydrodynamic Printed Sub-Microscale Fibrous Architectures [J]. | INTERNATIONAL JOURNAL OF BIOPRINTING , 2022 , 8 (2) : 1-12 . |
| MLA | Hu, Shugang et al. "Enhanced Attachment and Collagen Type I Deposition of MC3T3-E1 Cells via Electrohydrodynamic Printed Sub-Microscale Fibrous Architectures" . | INTERNATIONAL JOURNAL OF BIOPRINTING 8 . 2 (2022) : 1-12 . |
| APA | Hu, Shugang , Meng, Zijie , Zhou, Junpeng , Li, Yongwei , Su, Yanwen , Lei, Qi et al. Enhanced Attachment and Collagen Type I Deposition of MC3T3-E1 Cells via Electrohydrodynamic Printed Sub-Microscale Fibrous Architectures . | INTERNATIONAL JOURNAL OF BIOPRINTING , 2022 , 8 (2) , 1-12 . |
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Abstract ：
Additive manufacturing (AM) is considered the most promising technique to realize the in-situ fabrication of complex functional structures in space environments owing to its unique capability of full automation and digitalization. However, unlike printing on the earth, the zero/micro-gravity environment in space makes it extremely challenging to achieve precise deposition and stacking of the printed materials for microscale struc-tures. Here, we proposed to use high-voltage electrostatic force in the electrohydrodynamic printing (EHDP) process as the guiding force for the upward or anti-gravity (AG) printing of microscale functional structures, which can be potentially used for in-space environments. The average electrical field strength for stable AG-EHDP is found in the range of 1.70 x 107 V m-1 to 2.45 x 107 V m-1. In comparison with conventional EHDP, AG-EHDP can fabricate microscale 3D lattice structures with a smaller feature size of 3.65 +/- 1.31 mu m. A finite element analysis approach is established to simulate the AG-EHDP process at different parameters for the prediction of fiber width, which shows great consistency to the experimental results and applies to zero/micro-gravity printing environments. AG-EHDP also realizes the upward printing of silver microscale structures with a resistance of 7.72 +/- 0.83 omega cm-1, which exhibits the excellent electrothermal capability to heat the cold tem-plate from 25 degrees C to 180 degrees C within 3 min under an applied voltage of 3 V. It is foreseen that EHDP might provide a promising strategy to fabricate microscale functional structures in zero/micro-gravity space environments.
Keyword ：
Additive manufacturing Anti-gravity 3D printing Electrohydrodynamic printing In-space manufacturing Microscale structures
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| GB/T 7714 | Qu, Manqiang , Meng, Zijie , Gao, Tianjian et al. Exploration of electrohydrodynamic printing potentially for in-space fabrication of microscale functional structures: A preliminary study by an anti-gravity configuration [J]. | ADDITIVE MANUFACTURING , 2022 , 61 . |
| MLA | Qu, Manqiang et al. "Exploration of electrohydrodynamic printing potentially for in-space fabrication of microscale functional structures: A preliminary study by an anti-gravity configuration" . | ADDITIVE MANUFACTURING 61 (2022) . |
| APA | Qu, Manqiang , Meng, Zijie , Gao, Tianjian , He, Jiankang , Li, Dichen . Exploration of electrohydrodynamic printing potentially for in-space fabrication of microscale functional structures: A preliminary study by an anti-gravity configuration . | ADDITIVE MANUFACTURING , 2022 , 61 . |
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Abstract ：
Electric eels in nature can generate high voltage with hundreds of volts based on the mechanism of gradient induced ion flux, which provides an excellent prototype to inspire the exploration of more efficient and green energy generation strategies in artificial systems. Here we developed a novel microfluidics-based strategy to efficiently fabricate flexible ionic hydrogel batteries by mimicking ion-concentration gradients in biological organs. Microfluidic networks were designed to simultaneously transport four types of ionic hydrogel solutions into pre-defined well arrays and enable the perfusion of 801 hydrogel precursor wells within 1.53 min. A specifically designed photomask was used to selectively solidify these precursors into separate hydrogel particles, which were further assembled into a flexible ionic hydrogel battery pack by using negative pressure. The voltage output of the resultant ionic hydrogel battery pack was found to increase linearly with the increase of battery unit number, and a maximum voltage of 73.33 V can be achieved by connecting eight ionic hydrogel battery packs in series. The resultant ionic hydrogel battery exhibited unique capability in stably working in wide temperature ranges (0-90 degrees C) and large deformation conditions, and can be further preserved for a long period after dehydration and instantly recover its original discharge capacity once rehydrated. PEDOT:PSS was introduced to the ionic hydrogel battery for reduced internal resistance, resulting in a 1.5-fold improvement in electrical current output. The presented microfluidics-based and high-efficient fabrication strategy exhibits great promise to translate the biomimetic flexible power-generating systems into a viable soft power source for various electrically-driven applications.
Keyword ：
Electric eels Ion-concentration gradient Ionic hydrogel battery Microfluidics-based fabrication
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| GB/T 7714 | He, Pei , He, Jiankang , Huo, Ziyao et al. Microfluidics-based fabrication of flexible ionic hydrogel batteries inspired by electric eels [J]. | ENERGY STORAGE MATERIALS , 2022 , 49 : 348-359 . |
| MLA | He, Pei et al. "Microfluidics-based fabrication of flexible ionic hydrogel batteries inspired by electric eels" . | ENERGY STORAGE MATERIALS 49 (2022) : 348-359 . |
| APA | He, Pei , He, Jiankang , Huo, Ziyao , Li, Dichen . Microfluidics-based fabrication of flexible ionic hydrogel batteries inspired by electric eels . | ENERGY STORAGE MATERIALS , 2022 , 49 , 348-359 . |
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Abstract ：
Substantial challenges remain in constructing the native tendon-to-bone interface for rotator cuff heal-ing owing to the enthesis tissues' highly organized structural and compositional gradients. Herein, we propose to bioprint living tissue constructs with layer-specific growth factors (GFs) to promote enthesis regeneration by guiding the zonal differentiation of the loaded stem cells in situ . The sustained release of tenogenic, chondrogenic, and osteogenic GFs was achieved via microsphere-based delivery carriers em-bedded in the bioprinted constructs. Compared to the basal construct without GFs, the layer-specific tis-sue analogs realized region-specific differentiation of stem cells in vitro . More importantly, bioprinted living tissue constructs with layer-specific GFs rapidly enhanced the enthesis regeneration in a rabbit ro-tator cuff tear model in terms of biomechanical restoration, collagen deposition, and alignment, showing gradient interface of fibrocartilage structures with aligned collagen fibrils and an ultimate load failure of 154.3 +/- 9.5 N resembling those of native enthesis tissues in 12 weeks. This exploration provides a feasi-ble strategy to engineer living tissue constructions with region-specific differentiation potentials for the functional repair of gradient enthesis tissues.Statement of significancePrevious studies that employed acellular layer-specific scaffolds or stem cells for the reconstruction of the rotator cuff faced challenges due to their insufficient capability to rebuild the anisotropic compositional and structural gradients of native enthesis tissues. This manuscript proposed a living tissue construct with layer-specific, GFs-loaded mu S, which can direct in situ and region-specific differentiation of the em-bedded stem cells to tenogenic, chondrogenic, and osteogenic lineages for functional regeneration of the enthesis tissues. This bioprinted living tissue construct with the unique capability to reduce fibrovascular scar tissue formation and simultaneously facilitate enthesis tissue remodeling might provide a promising strategy to repair complex and gradient tissues in the future.(c) 2022 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Keyword ：
3D bioprinting Living tissue constructs Rotator cuff Zonal differentiation
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| GB/T 7714 | Bai, Lang , Han, Qian , Meng, Zijie et al. Bioprinted living tissue constructs with layer-specific, growth factor-loaded microspheres for improved enthesis healing of a rotator cuff [J]. | ACTA BIOMATERIALIA , 2022 , 154 : 275-289 . |
| MLA | Bai, Lang et al. "Bioprinted living tissue constructs with layer-specific, growth factor-loaded microspheres for improved enthesis healing of a rotator cuff" . | ACTA BIOMATERIALIA 154 (2022) : 275-289 . |
| APA | Bai, Lang , Han, Qian , Meng, Zijie , Chen, Baojun , Qu, Xiaoli , Xu, Meiguang et al. Bioprinted living tissue constructs with layer-specific, growth factor-loaded microspheres for improved enthesis healing of a rotator cuff . | ACTA BIOMATERIALIA , 2022 , 154 , 275-289 . |
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