Inflow　field　velocity　distributions　within　micro-channels　were　measured　by　Micro-PIV　technique.　Deionized　water　seeded　with　3μm-diameter　fluorescent　particles　was　introduced　into　micro-channel　pipeline.　The　flow　field　was　recorded　by　14-bit　CCD　camera　system　combining　10-fold　microscope.　Under　commercial　CFD　software　Fluent,　Realizable　k-Ε　model　with　porous　medium　model,　as　which　roughness　elements　of　micro-channel　wall　was　abstracted,　was　used　to　study　on　the　micro　channel　having　rectangular　cross-section　with　side　length　of　600μm　and　800μm,　respectively,　to　acquire　their　velocity　distribution　under　Re　of　100　and　300.　Compared　with　that　of　Micro-PIV　experiment,　the　simulation　result　indicated　that,　Realizable　k-Ε　model　with　porous　medium　model　could　effectively　simulate　fluid　flow　of　micro-scales　pipeline,　in　which　porous　medium＇s　thickness　was　converted　by　relative　roughness　in　the　micro-scales,　and　porous　medium＇s　viscosity　factor　and　inertia　coefficient　were　determined　by　fluid　state　and　resistance　within　porous　medium.