Under　Re　of　100　and　300　respectively,　the　flow　field　distributions　in　jagged　micro-channel　with　side　lengths　of　800　μm　were　investigated　by　experimental　measurement　and　numerical　simulation.　Applying　micro-PIV　technique,　the　research　got　the　velocity　vector　distributions　and　streamline　graphs　of　the　flow　field.　The　results　show　that　the　flow　structures　are　the　same　in　each　gear　of　the　micro-channel.　It　has　high-speed　zone　and　low-speed　zone　in　the　flow　field　of　single　gear,　and　the　backflow　appears　in　the　flat　top　and　corner　with　a　complete　vortex　in　the　low-speed　zone.　A　novel　numerical　method　for　simulating　micro-scale　flow　field　was　established　mainly　by　adopting　porous　medium　model,　realizable　k-Ε　turbulence　model,　and　single　gear　geometric　model　setting.　The　flow　field　distributions　in　the　micro-channel　are　got　by　simulating　and　calculating　under　periodic　boundary　conditions.　The　simulation　flow　fields　are　consistent　with　the　experimental　results.　Through　the　quantified　analysis　on　the　specific　cross　section,　it　proves　that　the　simulation　value　and　experimental　value　have　a　high　degree　of　similarity,　and　the　numerical　simulation　approach　can　be　used　in　all　the　inflow　velocity　simulation　for　the　complex　structured　micro-channels　with　a　wider　range　of　Reynolds.