As　a　typical　steroid　hormone　drug,　estradiol　(E2)　is　also　one　of　the　most　frequently　detected　endocrine　disrupting　chemicals　(EDCs)　in　the　aquatic　environment.　Herein,　in　response　to　the　potential　risk　of　E2　in　steroid　hormone　pharmaceutical　industry　wastewater　to　human　and　wildlife,　a　novel　carbon　nanotubes　/　amine-functionalized　Fe3O4　(CNTs/MNPs@NH2)　nanocomposites　with　magnetic　responsive　have　been　developed　for　the　enrichment　and　extraction　of　E2　in　pharmaceutical　industry　wastewater,　where　amino-functionalized　Fe3O4　magnetic　nanoparticles　(MNPs@NH2)　were　used　as　a　magnetic　source.　The　resultant　CNTs/MNPs@NH2　possessed　both　the　features　of　CNTs　and　desired　magnetic　property,　enabling　to　rapidly　recognize　and　separate　E2　from　pharmaceutical　industry　wastewater.　Meanwhile,　the　CNTs/MNPs@NH2　had　good　binding　behavior　toward　E2　with　fast　binding　kinetics　and　high　adsorption　capacity,　as　well　as　exhibited　satisfactory　selectivity　to　steroidal　estrogen　compounds.　Furthermore,　the　change　of　pH　value　of　aqueous　phase　in　adsorption　solvent　hardly　affected　the　adsorption　of　E2　by　CNTs/MNPs@NH2,　and　the　adsorption　capacity　of　E2　ranged　from　19.9　to　17.2　mg　g−1　in　the　pH　range　of　3.0　to　11.0,　which　is　a　latent　advantage　of　the　follow-up　development　method　to　detect　E2　in　pharmaceutical　industry　wastewater.　As　a　result,　the　CNTs/MNPs@NH2　serving　as　a　solid　phase　extraction　medium　were　successfully　applied　to　efficiently　extract　E2　from　pharmaceutical　industry　wastewater.　Therefore,　the　CNTs/MNPs@NH2　nanocomposites　could　be　used　as　a　potential　adsorbent　for　removing　steroidal　estrogens　from　water.　More　importantly,　the　developed　method　would　provide　a　promising　solution　for　the　monitoring　and　analysis　of　EDCs　in　pharmaceutical　industry　wastewater.　©　2021