Cardiovascular　diseases　continue　to　threaten　human　life,　health　and　safety.　Among　various　treatment　methods,　myocardial　tissue　engineering　is　one　of　the　important　methods　to　solve　the　problem　of　cardiovascular　disease.　The　main　goal　of　myocardial　tissue　engineering　is　to　make　the　synthetic　or　repaired　myocardium　have　its　original　physiological　function,　and　the　pattering　of　myocardium　cells　into　a　regular　directional　arrangement　is　one　of　the　most　challenging　techniques.　Therefore,　in　this　study,　a　surface　acoustic　wave　microfluidic　chip　with　LiNbO3　as　the　piezoelectric　substrate　was　designed　to　meet　the　needs　of　patterned　arrangement　of　cardiomyocytes.　Firstly,　this　study　introduces　the　preparation　method　of　the　microfluidic　chip　based　on　LiNbO3.　The　microfluidic　chip　is　used　to　carry　out　the　patterning　simulation　experiment　of　cardiomyocytes,　and　it　is　verified　that　the　surface　acoustic　wave　can　make　the　silica　particles　oriented　in　the　flow　channel.　The　micro-channel　is　used　to　simulate　the　growth　environment　of　cardiomyocytes.　Secondly,　by　analyzing　the　experimental　results,　it　is　concluded　that　the　surface　acoustic　wave　microfluidic　chip　designed　in　this　study　possesses　patterned　particles,　demonstrating　its　application　potential　in　repairing　the　damaged　surface　of　myocardial　tissue.　Finally,　a　surface　acoustic　wave　microfluidic　chip　based　on　LiNbO3　was　used　to　arrange　cardiomyocytes　in　the　hydrogel.　Scanning　the　solidified　myocardial　fibers,　it　is　concluded　that　the　directional　arrangement　of　the　internal　myocardial　cells　of　the　myocardial　fibers　obtained　from　the　experiment　is　consistent　with　the　theory.　Demonstrated　the　huge　application　potential　of　LiNbO3　substrate　surface　acoustic　wave　microfluidic　chip　for　cardiomyocyte　patterning　in　myocardial　tissue　engineering.　©　2022　ACM.