With　the　technology　development　of　cold　atmospheric　plasma　(CAP),　CAP　draws　more　and　more　attentions　to　the　biological　application　due　to　the　low　gas　temperature　and　high　activity　of　reactive　species　produced　by　CAP.　Plasma　medicine　was　also　established　as　an　innovative　interdisciplinary,　combining　with　the　plasma　physics,　chemistry,　life　sciences　and　clinical　medicine,　etc.　Here,　we　gave　a　brief　introduction　about　CAP,　its　generation　and　composition,　the　interaction　with　liquid　and　tissues,　and　some　of　the　major　applications　in　biomedical　field,　such　as　sterilization,　blood　coagulation,　wound　healing,　skin　disease　and　dental　treatment.　In　addition,　we　focused　on　the　application　of　CAP　in　cancer　treatment.　CAP　can　effectively　induce　tumor　cell　death,　inhibit　cell　proliferation　and　migration,　induce　differentiation　of　tumor　cells　and　suppress　the　potential　of　stem　cells,　and　can　increase　the　sensitivity　to　chemotherapy.　These　beneficial　effects　shows　a　promising　prospects　for　CAP　application　in　tumor　therapy.