Investigation　of　the　heat　transfer　in　biological　tissues　is　of　great　importance　during　the　laser-mediated　thermal　therapies　of　skin　diseases.　These　processes　generally　feature　rapid　heating　with　high　heat　flux　and　small　temporal　scales　within　extremely　heterogeneous　tissue　media.　Under　those　conditions,　the　non-Fourier　effect　should　be　taken　into　account.　This　paper　studied　the　bio-heat　transfer　in　skin　tissue　model　that　irradiated　by　pulsed　laser.　After　optical　transmission　and　energy　deposition　were　obtained　by　Monte　Carlo　method,　the　dual-phase-lag　(DPL)　model　was　employed　and　solved　by　using　a　three-level　finite　difference　method　to　characterize　non-Fourier　heat　conduction　by　focusing　on　the　thermal　lag　during　the　interaction　of　pulsed　laser　with　biological　tissues.　It　is　found　that　substantial　discrepancies　exist　among　the　temperature　responses　of　biological　tissues　with　and　without　considering　the　non-Fourier　effect.　The　calculated　temperature　in　the　nonFourier　heat　transfer　is　lower　and　the　appearance　of　the　highest　temperature　exhibits　lagging　behaviors.　©　2018,　Science　Press.　All　right　reserved.