Glioblastoma　multiforme　is　a　type　of　central　nervous　system　tumor　with　extremely　poor　prognosis.　Previously,　hydrogen　peroxide　(H2O2),　which　promotes　the　oxidative　stress　response,　has　been　reported　to　induce　the　apoptosis　of　glioma　cells.　Recently,　secreted　frizzled-related　protein　1　(SFRP1)　has　been　shown　to　be　associated　with　various　types　of　malignant　tumors　and　with　H2O2-induced　oxidative　stress　in　cardiomyocytes　by　negatively　regulating　the　Wnt　signaling　pathway.　This　study　aimed　to　explore　SFRP1　expression　and　its　roles　in　H2O2-induced　apoptosis　in　human　glioma　cells.　We　found　that　the　SFRP1　level　was　decreased　in　several　human　glioma　cell　lines,　including　U87,　U251,　and　SW1783　cells.　In　U251　cells,　SFRP1　could　function　as　a　cancer　suppressor　gene,　and　the　growth　of　U251　cells　could　be　inhibited　not　only　by　H2O2　but　also　by　the　overexpression　of　SFRP1.　Furthermore,　we　demonstrated　that　H2O2-induced　SFRP1　gene　demethylation　partially　contributed　to　H2O2-induced　U251　cell　apoptosis,　which　was　verified　by　studies　using　an　SFRP　inhibitor　(WAY-316606).　Our　research　identified　that　H2O2-induced　SFRP1　gene　demethylation　contributes　to　H2O2-induced　apoptosis　in　human　U251　glioma　cells.