The　development　of　ischemic　stroke　is　associated　with　advanced　age.　Telomere　length,　as　a　marker　of　biological　aging,　has　been　reported　to　influence　the　risk　of　several　age-related　diseases,　including　ischemic　stroke.　Recent　studies　have　identified　the　genetic　variant　within　ACYP2　and　TSPYL6　associated　with　shorter　telomere　length.　The　objective　of　this　study　is　to　investigate　the　putative　association　of　ischemic　stroke　with　common　polymorphisms　in　ACYP2　and　TSPYL6　genes　in　a　Chinese　Han　population.　We　found　that　the　risk　alleles　of　six　single　nucleotide　polymorphisms　(SNPs),　including　rs11125529,　rs12615793,　rs843711,　rs11896604,　and　rs843706　within　both　ACYP2　and　TSPYL6,　and　rs17045754　in　ACYP2　gene,　were　related　with　increased　risk　of　ischemic　stroke　according　to　both　allelic　and　genotype　association　analyses.　The　significant　correlations　between　ACYP2　and　TSPYL6　SNPs　and　ischemic　stroke　risk　were　also　observed　in　dominant,　recessive,　and　additive　models,　respectively.　Two　blocks　in　high　linkage　disequilibrium　were　identified　in　this　study,　and　two　haplotypes　were　associated　with　higher　ischemic　stroke　susceptibility.　In　conclusion,　the　genetic　polymorphisms　of　ACYP2　and　TSPYL6　are　associated　with　increased　risk　of　developing　ischemic　stroke.　Further　studies　with　larger　sample　sizes　are　required　to　validate　our　findings.