TMPRSS11D　is　a　member　of　the　type　II　transmembrane　serine　proteases　(TTSPs)　family　that　is　implicated　in　the　development　and　progression　of　several　cancers.　However,　the　biological　roles　of　TMPRSS11D　in　cervical　cancer　have　not　been　investigated.　In　the　present　study,　we　detected　the　expression　levels　of　TMPRSS11D　in　human　cervical　cancer　tissues　and　cell　lines.　The　results　showed　that　TMPRSS11D　expression　was　significantly　upregulated　in　cervical　cancer　tissues　as　compared　to　the　adjacent　normal　tissues.　Besides,　TMPRSS11D　was　highly　expressed　in　human　cervical　cancer　cell　lines.　Then　we　knocked　down　TMPRSS11D　in　cervical　cancer　cell　lines　to　evaluate　the　effects　of　TMPRSS11D　knockdown　on　cervical　cancer　cells.　The　results　showed　that　knockdown　of　TMPRSS11D　significantly　suppressed　cell　proliferation,　migration　and　invasion　in　cervical　cancer　cell　lines.　Furthermore,　the　data　revealed　that　TMPRSS11D　knockdown　prevented　epithelial-mesenchymal　transition　(EMT),　as　proved　by　the　increased　E-cadherin　expression,　as　well　as　decreased　N-cadherin　and　fibronectin　expressions.　Additionally,　knockdown　of　TMPRSS11D　inhibited　the　activation　of　the　PI3K/Akt　pathway　in　cervical　cancer　cells.　Furthermore,　insulin-like　growth　factor-1　(IGF-1)　treatment　reversed　the　inhibitory　effects　of　TMPRSS11D　knockdown　on　cell　proliferation　and　migration.　Collectively,　knockdown　of　TMPRSS11D　exerted　anti-tumor　activity,　at　least　in　part,　via　inhibiting　the　PI3K/Akt　pathway.　These　findings　indicated　that　TMPRSS11D　might　serve　as　a　novel　therapeutic　target　for　the　treatment　of　cervical　cancer.　©　2019　The　Royal　Society　of　Chemistry.