Liver　tissue　engineering　is　going　to　be　an　effective　treatment　for　end-stage　liver　disease.　In　this　work,　we　distributed　bone　marrow　mesenchymal　stem　cells　(BMSCs)　into　a　fast-forming　hydrogel　system　to　develop　a　liver-mimicking　construct　for　liver　regeneration.　The　advantage　of　this　hydrogel　system　was　that　this　BMSC-encapsulating　hydrogel　could　be　formed　via　a　bioorthogonal　reaction　between　2-cyanobenzothiazole　and　cysteine　within　several　seconds.　Thereafter,　we　explored　the　morphology,　biocompatibility,　and　expressions　of　hepatic　differentiation　markers　of　this　hydrogel　system.　These　results　illustrated　that　this　system　could　provide　a　suitable　niche　for　BMSC　proliferation　and　differentiation,　which　could　aid　in　future　biomedical　research　of　liver　regeneration.　©　2019　American　Chemical　Society.